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Home page > MLVA Web service > Frequently asked questions > Is it better to use agarose or capillary electrophoresis for MLVA typing?

Is it better to use agarose or capillary electrophoresis for MLVA typing?

The rule is to do what is most convenient for you. If you have access to a capillary electrophoresis sequencing machine, and anticipate to have a significant number of strains to type, then this is clearly the best option : you can multiplex the amplifications so that even a 20 loci MLVA assay can be run in less than five PCR amplifications per strain. Also allele calling is automated. Whatever applicable method you use, the end results will be identical. Applicable means that of course you cannot expect to type a 3 bp repeat unit locus on an agarose gel.