Help File
for the Legionella pneumophila MLVA typing page :
version 1, September 2006
The data which you can query is based upon Pourcel et al. JCM 2007. A total of 99 isolates from various origins have been genotyped at 10 tandem repeat loci. Only 8 markers are used in the clustering analyses; the Lpms31 and Lpms37 markers are highly variable and of interest for local outbreaks investigations.
This site assumes that you have determined the size of your PCR products, in the way which is most convenient for you (standard horizontal agarose gels, acrylamide gels, or using sequencing machines). We can provide upon request reference DNA to help set up and control your allele size assignments.
To help for
the allele assignation:
Below is
the list of sizes and copy
numbers observed so far among 99 isolates belonging to panel 1-3 and to the
proficiency panel (Pourcel et al. submitted):
|
(45-bp
repeat) |
Lpms3 (96-bp
repeat) |
Lpms13 (24-bp
repeat) |
Lpms17 (39-bp
repeat) |
Lpms19_b (21-bp
repeat) |
Lpms31 (45-bp
repeat) |
Lpms33 (125-bp
repeat) |
Lpms34 (125-bp
repeat) |
Lpms35 (18-bp
repeat) |
Lpms37 (7-bp
repeat) |
||||||||||
|
No.
of repeats |
Size
(bp) |
No.
of repeats |
Size
(bp) |
No.
of repeats |
Size
(bp) |
No.
of repeats |
Size
(bp) |
No.
of repeats |
Size
(bp) |
No.
of repeats |
Size
(bp) |
No.
of repeats |
Size
(bp) |
No.
of repeats |
Size
(bp) |
No.
of repeats |
Size
(bp) |
No.
of repeatsb |
Size
(bp) |
|
6 |
475 |
7 |
846 |
3 |
236 |
1.5 |
258 |
4 |
173 |
6 |
453 |
1 |
227 |
1 |
209 |
3 |
202 |
6 |
151 |
|
7 |
520 |
7.5 |
910 |
4 |
260 |
2 |
278 |
5 |
194 |
9 |
588 |
2 |
352 |
2 |
334 |
5 |
238 |
7 |
158 |
|
7.5 |
535 |
8 |
941 |
6 |
308 |
|
|
|
|
9.5 |
615 |
3 |
477 |
3 |
460 |
6 |
256 |
8 |
165 |
|
8 |
565 |
|
|
7 |
332 |
|
|
|
|
10 |
633 |
4 |
604 |
|
|
7 |
274 |
9 |
172 |
|
9 |
610 |
|
|
8 |
356 |
|
|
|
|
12 |
723 |
5 |
727 |
|
|
8 |
292 |
10 |
179 |
|
9.5 |
626 |
|
|
9 |
380 |
|
|
|
|
12.5 |
745 |
|
|
|
|
10 |
328 |
11 |
186 |
|
|
|
|
|
10 |
404 |
|
|
|
|
13 |
768 |
|
|
|
|
11 |
346 |
12 |
193 |
|
|
|
|
|
11 |
428 |
|
|
|
|
13.5 |
794 |
|
|
|
|
12 |
364 |
13 |
200 |
|
|
|
|
|
12 |
452 |
|
|
|
|
14 |
813 |
|
|
|
|
13 |
282 |
14 |
207 |
|
|
|
|
|
13 |
476 |
|
|
|
|
14.5 |
832 |
|
|
|
|
14 |
400 |
15 |
214 |
|
|
|
|
|
16 |
548 |
|
|
|
|
15 |
858 |
|
|
|
|
16 |
436 |
16 |
221 |
|
|
|
|
|
17 |
572 |
|
|
|
|
16 |
903 |
|
|
|
|
17 |
454 |
17 |
228 |
|
|
|
|
|
|
|
|
|
|
|
17 |
948 |
|
|
|
|
18 |
472 |
18 |
235 |
|
|
|
|
|
|
|
|
|
|
|
19 |
1038 |
|
|
|
|
21 |
526 |
20 |
249 |
|
|
|
|
|
|
|
|
|
|
|
20 |
1082 |
|
|
|
|
22 |
544 |
21 |
256 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
23 |
562 |
22 |
263 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
24 |
580 |
23 |
270 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
25 |
598 |
24 |
277 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
26 |
616 |
25 |
284 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
27 |
634 |
26 |
291 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
28 |
652 |
27 |
297 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
29 |
670 |
32 |
332 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
32 |
724 |
35 |
360 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
44 |
425 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
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|
a The philadelphia 1 alleles are indicated in bold
b The existence of 8bp repeats can only be assessed by sequencing
Pathogenic Bacteria Strain Typing, Université Paris Sud 91405 Orsay,
France.
Phone: +33 1 69 15 62 08, fax: +33 1 69 15 66 78. E-mail: minisatellites.