Methodology


Genotyping
DNA preparation
For PCR, DNA can be purified using commercial kits or alternatively thermolysates are prepared by heating bacteria in TE for 20min at 95°C then cooling in ice and centrifugation. The supernatant is kept frozen at -20°C.

PCR-based methods
DR analysis
MLVA 16
RD 105
Other techniques
IS110 RFLP