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Home page > MLVA Web service > Frequently asked questions > I do not have the 16M reference strain. Is it possible to use an other control strain ?

I do not have the 16M reference strain. Is it possible to use an other control strain ?

Any strain can be used. A vaccine strain is convenient, Rev1, S19, RB51, since typing data for representatives is available from Le Flèche 2006 supplementary file. For instance, if willing to use the abortus S19 vaccine strain as a reference : first find what genotype is expected for strain S19. Go to Le Flèche 2006, get supplementary data (text) file, search for S19, (genotype 161) the panel 1 typing result is :

4-5-4-12-2-2-3-3

Then using the allele conversion table and the published primers, the expected genotype can be converted into expected sizes for the S19 strain : Bruce06 542 bp Bruce08 366 Bruce11 383 Bruce12 377 Bruce42 289 Bruce43 182 Bruce44 151 Bruce45 273

(panel 2 sizes can be deduced in the same way). It is recommended to eventually sequence the alleles for the local S19 strain, since one of the alleles might have mutated especially at the most variable loci in panel 2B.