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Section :
In the headlines
Articles and subsections :
Become a curator of the site
Section :
To be noted
Articles and subsections :
2016, May 19th, major
Brucella
database update
2018,
Brucella
version 4_3 database update
Brucellosis 2008
First MLVA ring trial : join now !
Second MLVA ring trial : join now !
Section :
News
Articles and subsections :
2016, May 16th, MicrobesGenotyping site released for beta-testing
Brucella microti
isolated from red fox in Austria
Brucella microti
isolated from soil
Brucella2009
just released
Brucella
2010 released
Brucella
2012 released
Diversity of clinical
Brucella melitensis
isolates in Lebanon
MLST investigation within the
Brucella
genus
MLVA analysis of
Brucella
isolates from human patients
MLVA for
Brucella
by Le Flèche et al., 2006
MLVA for
Brucella
by Whatmore et al., 2006
MLVA ring trial 2007, current list of participants
MLVA ring trial announcement : closing time
MLVA typing of
Brucella
isolates from humans in Sicily, Italy
New species
Brucella microti
validly published
Stability of MLVA loci in the
Brucella melitensis
Rev1 vaccine strain
The
Brucella2007
MLVA database just released
Two new species within the
Brucella
genus
Section :
Curators
Articles and subsections :
Le Flèche Philippe
Melzer Falk
Neubauer Heinrich
Vergnaud Gilles
Walravens Karl
Whatmore Adrian
Section :
MLVA ring trials
Articles and subsections :
MLVA ring trial 2007
Section :
MLVA Web service
Articles and subsections :
Brucella
MLVA database
Access the
Brucella
MLVA database
Frequently asked questions
Do I need to do a PCR on the control strain each time a PCR for an "unknown" strain is setup? Or could I simply use leftover product from a previous PCR on the control strain to run on a gel with the products from the strains I am trying to type ?
I can get the PCR assay to work, but am having problems analyzing the bands to get accurate sizes from my gels. I wonder how to actually calculate an exact band size and the repeat number. I am using the size markers to make a standard curve based on distance migrated on the gel. This old fashioned method is working, but once I get a band size, I cannot figure out how to get a repeat copy number.
I do not have the 16M reference strain. Is it possible to use an other control strain ?
Is it better to use agarose or capillary electrophoresis for MLVA typing?
May I run different loci on the same agarose gel ?
Utilities (protocols, etc.)
Brucella
TR names
Protocol for agarose-gel typing set-up
Table for allele assignement
Section :
Related links
Articles and subsections :
MLVA-NET for
Pseudomonas aeruginosa
Link to Web page for
Pseudomonas
Our home page
Link to GPMS home page
Tandem repeats database
Link to WebPage for tandem repeats
The CRISPR WebService
Link to the CRISPR WebService
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